Brand: In-Vitro Technologies
LiveBox2 is a transparent chamber, developed to model physiological barriers (e.g. lung and intestinal epithelium) in-vitro.
LiveBox2 is a transparent chamber, developed to model physiological barriers (e.g. lung and intestinal epithelium) in-vitro. LB2 is designed for inter-connected dynamic cell cultures and is equipped with two flow inlets and outlets, and a holder to house a porous membrane. The clamp system provided with the LB2 assures the watertight closure of the system in both static and dynamic conditions (up to 0.5 mL/min in apical or basal compartments).
- 1 LB2 equipped with glass discs
- 1 clamp systems
- PDMS tubes, luer lock connectors, and 2 mixing chambers to realize two fluidic circuits
- 1 extractor
- The system is designed to reproduce the typical surface of a 12 transwell.
- The removable transparent glass bottom allows for:
- Live imaging during static/dynamic culture, i.e. in situ imaging of the three-dimensional culture environment using optical microscopy.
- The removable holder system allows for:
- Housing of a porous membrane
- Easy and safe handling of the membrane where cells are seeded
- Post culture imaging including staining procedures or any other sample processing. After disassembling the LB2, the holder system supporting the cultivated cell monolayer or 3D construct can be easily removed from the bottom part and used for further investigations and analysis.
- Modularity: LB2 can be connected with other cell culture systems in order to mimic cross-talk between tissues (multi-organ simulation).
One of the microfluidic systems that can be used to study drug transport across the BBB is the LiveBox platform from IVTech, a company based in Italy that was spun out from the University of Pisa. The commercially available LiveBox2 system has two chambers separated by a membrane and each chamber has independent inlet and outlet systems. The system can be configured to study perfusion from one chamber to another across the endothelial cell layer on the membrane. The glass-bottom culture well allows live imaging as well as fixation and staining of the cells for tight junction and other markers at the end of the experiment. Currently, the LiveBox2 system does not include electrodes to measure TEER but future versions can include voltage and current electrodes in each chamber to measure TEER. This development will be essential to measure key BBB endpoints in a 3D model with fluid flow.