PerfusionPal Starter System


  • Brand: Lena Biosciences
The starter system enables continuous perfusion of 12 or 48 statistically independent organ cultures with one plate.

Lena Biosciences PerfusionPal 3D cell culture with perfusion and a proprietary blood substitute bring life to your cells.

3D biological systems are closer to real physiology than simplistic model systems such as 2D monocultures due to presence of cell-to-cell contacts, extracellular matrix (ECM), and cellular heterogeneity. Fidelity of 3D model cultures to biological reality is currently limited by availability of screening platforms with fluidic perfusion to control cellular microenvironment. A new platform that fills this gap is available from Lena Biosciences. PerfusionPal 3D an enabling microphysiological systems (MPS) with significantly increased translational value for drug screening compared with platforms that lack perfusion. The PefusionPal 3D comes with plates with 12- or 48-wells, which allows this platform to easily fit the existing workflow for drug development, including liquid handling automation.

PerfusionPal 3D works together with other proprietary products from Lena Biosciences, including blood substitute regent, and cell-seeding scaffolds SeedEZ and GradientEZ.

Note that the SeedEZ scaffolds for use with the PerfusionPal are specifically sized to the plates. Our other SeedEZ scaffolds are sized to fit in standard multi-well plates (96-well to 6-well). The two GradientEZ products (lobe and petal shapes) are for use in multi-well plates.
The starter system includes:
  • One dual syringe pump
  • Two 48-well PerfusionPal plates (or two 12-well plates)
  • Two syringes and tubing
  • 120 SeedEZ scaffolds
  • Two bottles of 100 mL blood substitute
Apart from the syringe pump, all components of this starter system are sterile. The main functions are to enable parallel perfusion and provide superior gas exchange to the basal side of each culture. The perfusion rate can be easily changed or paused for media changes.
The blood substitute does not support the growth of microorganisms, and it is immiscible with and insoluble in media, reagents, and drugs. It’s the only chemical in the syringes and tubing, so the risk of contamination is negligible compared to other perfusion platforms that utilize the culture media in tubing and syringes.

PerfusionPal 3D enables microphysiological systems with increased translational value
The starter system includes a dual syringe pump, two 48-well perfusion plates and a proprietary blood substitute. Place the pump outside the incubator and you are ready to go.
The starter system replaces 96 chips, at least 192 tubes, 96 media bottles and 95 pumps.
Use case:
Assessing drug metabolism in vitro can be very challenging as the gold standard of primary human hepatocytes (PHH) do not maintain significant function of their drug metabolizing CYP450 enzymes for more than a few hours. The resulting problem is that it is difficult, if not impossible to detect metabolites of many drugs in vitro. We have found that our PerfusionPal with blood substitute significantly increases CYP450 enzyme activity in PHH and helps to maintain it for longer in culture. We have also found this to be the case with iPSC-derived hepatocytes, HepaRG cells, and Hep G2 cells. We assess this metabolic activity using standard CYP450 Glo assays from Promega that produce a luminescent product which is sampled from the medium and read in a plate reader.
Some of the considerations for proper growth are:

1. Oxygen concentration, nutrients, shear flow, and bioactive components in the circulating fluid.
Oxygen and nutrient availability are the most critical factors in maintaining 3D cell cultures. As the cells grow denser, without perfusion, a culture can develop a necrotic core. The flow provided by the pumped blood substitute helps to circulate the medium and keep the cells supplied with nutrients. The blood substitute is a pure, synthetic compound containing only a single molecule (contains no bioactive components). It is twice as dense as water and is insoluble in and immiscible with culture media, reagents, and drugs. Cell culture medium floats on top of this liquid and pumping it in and out of the plate causes the cell culture medium to rise and fall. Nothing from the liquid phase of the medium enters the blood substitute even though they are in direct contact. The nature of the blood substitute allows for the dissolution of atmospheric gases at levels that are orders of magnitude higher than in cell culture medium. Oxygen and carbon dioxide are both very soluble in the blood substitute which means it acts as a secondary gas interface under the 3D culture, providing improved oxygenation and pH stability for bicarbonate-based buffer systems.
A big advantage of PerfusionPal over organ-on-a-chip systems is that our platform delivers in-well perfusion, ensuring that growth factors, as well as autocrine and paracrine signaling molecules are not being washed away. Additionally, this allows for the concentration of molecules that the cells are secreting, significantly improving the end-user’s ability to detect them in a timely fashion.
The following animation shows how the system works:

2. Additional considerations for users: culture duration
We have grown 3D cultures for months in our PerfusionPal system. The viability and function remain high for the duration of the experiment. We have not yet tested the limit of how long the cultures can survive as we had pre-determined experimental end points. The cultures are maintained similar to how they would be in a multi-well plate. Cell culture medium is replaced at regular intervals using a micropipet.

3. Sample amount :
The volume of medium in the PerfusionPal wells is 750 uL for the 12-well system and 300 uL for the 48-well system. This is more than enough to run nearly any type of assay that uses a plate reader for colorimetric, fluorometric, or luminescent readouts. The medium sampled is also suitable for immuno-based assays such as western blotting and ELISA. Assays. Mix-and-measure assays that involve exposing the cells to a reagent and subsequently sampling and analyzing the medium can be done directly in the PerfusionPal. Alternatively, the scaffolds containing the cells may be transferred to a multi-well plate for assaying. The cells can be paraformaldehyde fixed and processed for immunofluorescent staining directly in the scaffolds. The cells can also be removed from the scaffolds, if necessary. Here is a link to a list of assays that we routinely run: and cell types that we use:

Benefit Detail
Flexibility Scaffolds can be removed for cell isolation and imaging, provides extended mechanistic studies, can be used for repeat drug dose testing
Affordability Cheaper than conventional relay assays
Throughput In-well assaying, provides superior gas exchange, users determine customizable perfusion rates
Special features

0 reviews for this product